ELISA試劑盒標本保存知多少

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">標本保留，在</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒試驗中是非常重要的，常有</font><font face="Calibri">ELISA</font><font face="宋體">操作員反映在標本保留時呈現溶血、凝聚不全、受細菌污染等景象發生，我公司技術員特對于有關常見問題做出總結。</font></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">下面咱們就來看看今日的內容：搞定</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒辦法我有妙招！</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">一、標本保留不當</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在冰箱中保留過久的標本，血清中</font>IgG<font face="宋體">可聚組成多聚體、</font><font face="Calibri">AFP</font><font face="宋體">可構成二聚體，在間接法</font><font face="Calibri">ELISA</font><font face="宋體">測定中會致使本底過深、乃至構成假陽性；標本放置時刻過長（如一天以上），有時抗原或抗體免疫活性減弱，亦可呈現假陰性。為戰勝上述攪擾，</font><font face="Calibri">ELISA</font><font face="宋體">測定的血清標本宜為新鮮收集；如不能當即測定，</font><font face="Calibri">5</font><font face="宋體">天內測定的血清標本可存放于</font><font face="Calibri">4</font><font face="宋體">℃，</font><font face="Calibri">1</font><font face="宋體">周后測定的血清標本應低溫凍存；凍存后融解的標本，蛋白質局部濃縮，散布不均，應充沛混合后再測定，但混勻時應輕柔，不行激烈振動。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">二、標本溶血</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒因為各種人為原因致使的標本溶血，均可因紅細胞損壞溶解時釋放出很多具有過氧化物酶活性的血紅蛋白，在以辣根過氧化物酶為符號的</font><font face="Calibri">ELISA</font><font face="宋體">測定中，會致使非特異性顯色，</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒攪擾測定成果。為戰勝上述攪擾效果，標本收集時有必要留意防止溶血。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">三、標本凝聚不全</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在沒有促凝劑和抗凝劑存在的狀況下，正常血液收集后</font>1/2~2h<font face="宋體">開端凝結，</font><font face="Calibri">18~24h</font><font face="宋體">徹底凝結。臨床查驗工作中，有時為了爭取時刻迅速檢測，常在血液還未開端凝結時即強行離心別離血清，此刻的血清中仍殘留部分纖維蛋白原，在</font><font face="Calibri">ELISA</font><font face="宋體">測定過程中能夠構成肉眼可見的纖維蛋白塊，易構成假陽性成果；這類狀況于次日復查時因血凝已徹底，血清中不再有纖維蛋白原存在，故復查成果變為陰性。為防止上述攪擾效果，解決的辦法最好是血液標本收集后有必要使其充沛凝結后再別離血清，或標本收集時用帶別離膠的采血管或于采血管中參加適當的促凝劑。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">四、標本管中添加物質的影響</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">抗凝劑、酶抑制劑及迅速別離血清的別離膠等均對</font>ELISA<font face="宋體">測定有一定攪擾效果。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">五、標本受細菌污染</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">因菌體中可能富含內源性辣根過氧化物酶，因而，被細菌污染的標本同溶血標本一樣，亦可產生非特異性顯色而攪擾測定成果。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">做好了</font> <strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">要素和操作要素以后，保證檢測成果的準確性就簡單的多了。我公司試劑盒供給全程輔導服務，為您的試驗擔任究竟！歡迎您來電咨詢我司業務員。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>