ELISA試驗“溫育”操作是不是只能在37℃條件下完結

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育是</font><strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">測定中影響測定勝敗最為關鍵的一個要素。</font><font face="Calibri">ELISA</font><font face="宋體">實驗作為一種固相免疫測定，抗原抗體的聯絡反應在固相上進行，要使液相中的抗原或抗體與固相上的特異抗體或抗原徹底聯絡，必須在一定的溫度條件下反應一定的時間。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育所需時間與溫度成反比，即溫度越高，則所需時間相對較短。在</font>ELISA<font face="宋體">中一般有兩次抗原抗體反應，即加標本和加酶聯絡物后。抗原抗體反應的完結需要有一定的溫度和時間，這一保溫進程稱為溫育</font><font face="Calibri">(incubation)</font><font face="宋體">，也被稱之為孵育。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">這是一個逐漸平衡的進程，因而需經渙散才華到達反應的結尾。在這今后參加的酶符號抗體與固相抗原的聯絡也相同如此。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育常選用的溫度有</font><font face="Calibri">43</font><font face="宋體">℃、</font><font face="Calibri">37</font><font face="宋體">℃、室溫文</font><font face="Calibri">4</font><font face="宋體">℃（冰箱溫度）等。</font><font face="Calibri">37</font><font face="宋體">℃是實驗室中常用的保溫溫度，也是大多數抗原抗體聯絡的適宜溫度。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">實驗標明，兩次抗原抗體反應一般在</font>37<font face="宋體">℃經</font><font face="Calibri">1-2</font><font face="宋體">小時，產物的生成可達高峰。為加快反應，可前進反應的溫度，</font><strong><font face="Calibri">ELISA</font><font face="宋體">試劑盒</font></strong><font face="宋體">有些實驗在</font><font face="Calibri">43</font><font face="宋體">℃進行，但不宜選用更高的溫度。抗原抗體反應</font><font face="Calibri">4</font><font face="宋體">℃更為徹底，在放射免疫測定中多使反應在冰箱中放置一晚上，以構成最多的堆積。但因所需時間太長，在</font><font face="Calibri">ELISA</font><font face="宋體">中一般不予選用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>