ELISA試劑盒技術(shù)的可操作性強

<div> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在通常的概念里，不需雜亂設(shè)備，甚至徹底手藝加樣、洗板和肉眼判讀成果，便可完結(jié)該技能的操作。隨著大家對質(zhì)量操控認(rèn)識的加強，盡也許做到最低極限的削減系統(tǒng)誤差，以及削減勞動強度等觀念的不斷改進，處理</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒技能中加樣、溫育、洗板及判讀成果進程的系統(tǒng)誤差疑問及高效率運作疑問致使了自動化概念的形成。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">技能的加樣、溫育、洗板及判讀成果進程的科學(xué)地、有機地、系統(tǒng)地聯(lián)系，盡也許地削減各環(huán)節(jié)的人為因素的影響，便成為自動化</font><font face="Calibri">ELISA</font><font face="宋體">技能的理論基。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">血漿中除尚富含纖維蛋白原和抗凝劑外，其他成份均平等于血清，制備血漿標(biāo)本需借助于抗凝劑，而血清標(biāo)本只需待血清天然凝結(jié)</font><font face="Calibri">,</font><font face="宋體">血塊收縮后即可獲得。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">除特殊情況外，在醫(yī)學(xué)查驗中均以血清作為檢查標(biāo)本在</font>ELISA<font face="宋體">試劑盒中血漿和血清可平等使用，血清標(biāo)本可按慣例辦法收集</font><font face="Calibri">,</font><font face="宋體">應(yīng)留意防止溶血</font><font face="Calibri">,</font><font face="宋體">紅細(xì)胞溶解時會釋放出具有過氧化物酶活性的物質(zhì)</font><font face="Calibri">,</font><font face="宋體">以</font><font face="Calibri">HRP</font><font face="宋體">為符號的</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒測定中</font><font face="Calibri">,</font><font face="宋體">溶血標(biāo)本也許會添加非特異性顯色。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">如有細(xì)菌污染，菌體中也許富含內(nèi)源性</font><font face="Calibri">HR</font><font face="宋體">也會發(fā)作假陽性反應(yīng)</font><font face="Calibri">.</font><font face="宋體">如在冰箱中保存過久，其間的可發(fā)作聚合</font><font face="Calibri">,</font><font face="宋體">在間接法</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒中可使本底加深</font><font face="Calibri">.</font><font face="宋體">通常說來，在</font><font face="Calibri">5</font><font face="宋體">天內(nèi)測定的血清標(biāo)本可放置于</font><font face="Calibri">4</font><font face="宋體">℃。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">超越一星期測定的需低溫冰存、凍住血清融解后，蛋白質(zhì)部分濃縮散布不均，應(yīng)充沛混勻宜輕緩</font>,<font face="宋體">防止氣泡</font><font face="Calibri">,</font><font face="宋體">可上下顛倒混和。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> </div> <p>&nbsp;</p>