操作ELISA試劑盒各個(gè)步驟的注意要點(diǎn)

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">在運(yùn)用前與室溫平衡，再進(jìn)行測(cè)定。在</font>ELISA<font face="宋體">試劑盒中通常有兩次抗原抗體反響，即加標(biāo)本和加酶聯(lián)系物后。抗原抗體反響的完結(jié)需要有必定的溫度和時(shí)刻，這一保溫進(jìn)程稱為溫育（</font><font face="Calibri">incubation</font><font face="宋體">），有人稱之為孵育，在</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒中似不恰當(dāng)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒屬固相免疫測(cè)定，抗原、抗體的聯(lián)系只在固相表面上發(fā)作。以抗體包被的夾心法為例，參加板孔中的標(biāo)本，其間的抗原并不是都有均等的和固相抗聯(lián)系的時(shí)機(jī)，只要最靠近孔壁的一層溶液中的抗原直接與抗體接觸。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">假如對(duì)試劑預(yù)備不太注意，在試驗(yàn)時(shí)將試劑從冰箱中拿出來(lái)即用，而疏忽了這種做法有可能影響后邊溫育時(shí)刻不行的疑問(wèn)影響后邊的操作。測(cè)定中試劑的預(yù)備最為要害的是，在試驗(yàn)開(kāi)端前，將試劑盒先從冰箱中拿出來(lái)，在室溫下放置</font>20min<font face="宋體">以上后，以使試劑盒</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋體">當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，防止起泡。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3. <font face="宋體">洗刷進(jìn)程十分重要，不充分的洗刷易形成假陽(yáng)性。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4. <font face="宋體">一次加樣時(shí)刻最佳操控在</font><font face="Calibri">5</font><font face="宋體">分鐘內(nèi)，如標(biāo)本數(shù)量多，引薦運(yùn)用排槍加樣。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5. ELISA<font face="宋體">試劑盒請(qǐng)每次測(cè)定的一起做規(guī)范曲線，最佳做復(fù)孔。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">6. <font face="宋體">如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高，請(qǐng)先稀釋后再測(cè)定。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">7. <font face="宋體">在制造規(guī)范品、檢查溶液工作液時(shí)，請(qǐng)以相應(yīng)的稀釋液制造，</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒不能混雜。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">8. <font face="宋體">底物請(qǐng)避光保留。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">9<font face="宋體">、操控好每一步的操作時(shí)刻。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">這些年，科研市場(chǎng)上各種投合花費(fèi)趨勢(shì)的商品層出，可是據(jù)有關(guān)查詢計(jì)算，在這些紛繁復(fù)雜的試劑商品背后，卻表現(xiàn)的是商品質(zhì)量的疑問(wèn)。商品就是實(shí)力的科研市場(chǎng)上，無(wú)論是品牌建設(shè)仍是途徑拓展，優(yōu)異的商品都是十分重要的。不光著重方式，還十分重視實(shí)質(zhì)的開(kāi)展形式。現(xiàn)在的</font>ELISA<font face="宋體">試劑盒花費(fèi)主體中主要有學(xué)校、科研所，商品市場(chǎng)上的需要也在逐步開(kāi)展。我公司當(dāng)下客戶來(lái)自四面八方，也有不少是試驗(yàn)菜鳥(niǎo)，甚至是學(xué)生。依據(jù)客戶的地域、時(shí)刻、試驗(yàn)方法的不一樣請(qǐng)求，我公司專業(yè)的技術(shù)人員會(huì)活躍調(diào)整，合作客戶。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">我公司</font>ELISA<font face="宋體">試劑盒長(zhǎng)久以來(lái)得到了客戶們的認(rèn)可與支持，今日圍繞著群眾反響分析評(píng)論我司</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒商品的作用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">對(duì)于客的效勞需要，咱們活躍調(diào)整</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">客戶的反響計(jì)算看出，質(zhì)量、效勞、報(bào)價(jià)、包裝等方面是對(duì)</font>ELISA<font face="宋體">試劑盒商品信任的主要原因。您的支持咱們會(huì)牢記于心，謝謝一路由您的相伴！讓您共完結(jié)試驗(yàn)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>