ELISA試劑盒常見問題

<div> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;"><font face="宋體">這里就簡單整理了</font>5<font face="宋體">個問題，具體內容如下：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋體">、一個</font><strong><font face="Calibri">ELISA</font><font face="宋體">試劑盒</font></strong><font face="宋體">檢測幾個樣品？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">我司在各大論壇看到最多的三個答案：</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋體">）</font><font face="Calibri">48T </font><font face="宋體">可做</font><font face="Calibri">42</font><font face="宋體">個樣本加</font><font face="Calibri">6</font><font face="宋體">個標準曲線；</font><font face="Calibri">96T </font><font face="宋體">可做</font><font face="Calibri">90</font><font face="宋體">個樣本加</font><font face="Calibri">6</font><font face="宋體">個標準曲線</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋體">）以</font><font face="Calibri">96T</font><font face="宋體">試劑盒來算，定量的試劑盒一般可以做</font><font face="Calibri">90</font><font face="宋體">個左右的樣品，定性的試劑盒可以做</font><font face="Calibri">94</font><font face="宋體">個左右的樣品</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋體">）</font><font face="Calibri">96T</font><font face="宋體">的</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒，去除標準品復孔檢測，最多還能檢測</font><font face="Calibri">80</font><font face="宋體">個標本；</font><font face="Calibri">48T</font><font face="宋體">的最多</font><font face="Calibri">30</font><font face="宋體">個樣本。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋體">、</font><font face="Calibri">elisa</font><font face="宋體">試劑盒</font><font face="Calibri">96T</font><font face="宋體">的能做檢測多少血清樣品？板能重復利用嗎？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">96T<font face="宋體">，一般做復孔的話，做標準曲線需要</font><font face="Calibri">16</font><font face="宋體">個孔，剩下</font><font face="Calibri">80</font><font face="宋體">個孔就可以做</font><font face="Calibri">40</font><font face="宋體">個樣本。這個板不能重復利用。而且配套的試劑也只是夠一塊板子用的。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋體">、</font><strong><font face="Calibri">ELISA</font><font face="宋體">試劑盒想</font></strong><font face="宋體">請教一下，我想用</font><font face="Calibri">elisa</font><font face="宋體">法測血清里的一種抗原，查了資料，發現沒有現成的試劑盒用，現在就有</font><font face="Calibri">2</font><font face="宋體">種備選方案，一種是買抗體對自己包被（查了</font><font face="Calibri">R&amp;D</font><font face="宋體">等大公司都沒有可用于</font><font face="Calibri">elisa</font><font face="宋體">的單抗）。哪種比較好呢？自己包被的會不會比國產的試劑盒可靠呢？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">如果你覺得技術不錯有隊其他公司不放心，可以自己購買抗體對自己包被，這個就是麻煩點，自己包被用著放心，舒心；</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4<font face="宋體">、</font><font face="Calibri">western </font><font face="宋體">和</font><font face="Calibri">ELISA </font><font face="宋體">處理樣本有何差別？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">Western Blot <font face="宋體">樣品制備要加蛋白裂解液（含有蛋白酶抑制劑），然后冰上研磨，收集于</font><font face="Calibri">EP</font><font face="宋體">管，冰上裂解</font><font face="Calibri">30min</font><font face="宋體">，</font><font face="Calibri">4</font><font face="宋體">度</font><font face="Calibri">12000rpm</font><font face="宋體">離心</font><font face="Calibri">15min</font><font face="宋體">，取上清分裝，即得到蛋白樣品。建議直接電泳，多余的</font><font face="Calibri">-80</font><font face="宋體">保存。</font><font face="Calibri">ELISA</font><font face="宋體">試劑盒建議分裝的蛋白每支略多于一次上樣量，一次性用完一支，避免反復凍融。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">樣品，直接腦組織冰上勻漿，收集于</font><font face="Calibri">EP</font><font face="宋體">管，同上一樣離心，大約</font><font face="Calibri">220-250ul</font><font face="宋體">每支分裝（因為</font><font face="Calibri">ELISA</font><font face="宋體">上樣量基本都是每孔</font><font face="Calibri">100ul</font><font face="宋體">，做復孔，每個樣品</font><font face="Calibri">2</font><font face="宋體">孔，就需要</font><font face="Calibri">200ul</font><font face="宋體">，為了避免不夠</font><font face="Calibri">2</font><font face="宋體">孔，分裝時要大于</font><font face="Calibri">200ul</font><font face="宋體">每支），直接實驗。多余的</font><font face="Calibri">-80</font><font face="宋體">保存，一樣避免反復凍融。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">不過</font>ELISA<font face="宋體">最好少量樣品進行預實驗，或者查閱文獻，看看大概組織里有多高濃度，選擇合適的試劑盒，測量范圍要與組織濃度相符。避免濃度過高測量不準，或者濃度過低，小于試劑盒最低可測濃度。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> </div>