ELISA試劑盒的血清標(biāo)本的收集

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">因此</font><strong><font face="宋體">在</font>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">測(cè)定中試劑的預(yù)備最為樞紐的是，在實(shí)驗(yàn)開始前，將試劑盒先從冰箱中拿出來，在室溫下放置</font><font face="Calibri">20</font><font face="宋體">分鐘以上后，再進(jìn)行測(cè)定，以使試劑盒在使用前與室溫平衡。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">加血清樣本及反應(yīng)試劑在現(xiàn)在的</font><strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">中，血清樣本的加入幾乎是獨(dú)一的要使用微量加樣器加入樣本的步驟。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育是</font>ELISA<font face="宋體">測(cè)定中影響測(cè)定成敗最為樞紐的一個(gè)因素。目前臨床上使用血清標(biāo)本測(cè)定的標(biāo)志物一般有傳染性病原體的抗原和抗體、腫瘤標(biāo)志物、激素、特種蛋白、細(xì)胞因子和治療藥物等。試劑的加入在國(guó)產(chǎn)試劑盒中基本上均是從滴瓶中滴加，除了要留意滴加的角度外，滴加的速度也很重要，滴加太快，很輕易泛起重復(fù)滴加或加在兩孔之間的現(xiàn)象，這樣就會(huì)在孔內(nèi)的非包被區(qū)泛起非特異吸附，從而引起非特異顯色。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">如可的松在早晨</font>4<font face="宋體">～</font><font face="Calibri">6</font><font face="宋體">點(diǎn)之間，會(huì)有一峰值泛起：生長(zhǎng)激素、促黃體激素（</font><font face="Calibri">LH</font><font face="宋體">）和促卵泡激素（</font><font face="Calibri">FSH</font><font face="宋體">）均以陣發(fā)性方式開釋。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">因此，在測(cè)定此類激素時(shí)，</font><strong>ELISA<font face="宋體">試劑盒</font></strong><font face="宋體">要是為了在后面的溫育反應(yīng)步驟中，能使反應(yīng)微孔內(nèi)的溫度能較快地達(dá)到所要求的高度，以知足測(cè)定要求。再如治療藥物的檢測(cè)，應(yīng)根據(jù)藥代動(dòng)力學(xué)選擇服藥后的最適時(shí)間抽血檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育所需時(shí)間與溫度成反比，即溫度越高，則所需時(shí)間相對(duì)較短。使用微量加樣器加樣必需留意的樞紐點(diǎn)是：加樣不可太快，要避免加在孔壁上部，</font>ELISA<font face="宋體">試劑盒不可濺出和產(chǎn)氣憤泡。對(duì)用于激素和治療藥物測(cè)定，要留意收集時(shí)間甚或體位有可能會(huì)對(duì)測(cè)定結(jié)果產(chǎn)生影響。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>