酶聯生物與您嘮嘮關于抗體特異性的選擇

<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">陽春五月，<span style="color: rgb(255, 0, 0);"><strong>酶聯生物</strong></span>與您嘮嘮關于抗體特異性的挑選。特異性的挑選首要需求考慮四個方面：蛋白特異性、種屬特異性、試驗辦法特異性、符號物的特異性。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">1<font face="宋體">、蛋白特異性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">針對需求檢測的蛋白查找抗體，幾個細節要區別，重組表達的蛋白和內源性蛋白的檢測，對抗體的要求是不一樣的，留意檢查抗體闡明書的檢測闡明。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">假如重組蛋白不</font> <font face="宋體">是全長表達，則需求留意抗體的免疫原區域是否在重組蛋白區域內。內源性蛋白能清楚其剪切與潤飾的方式，特別表型的蛋白需求進行序列比對，并結合抗體免</font> <font face="宋體">疫原序列，檢查穿插反響的狀況。磷酸化蛋白檢測需求斷定詳細位點，不同位點的磷酸化意味著可能有不同的機制存在，不宜一概而論。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">2<font face="宋體">、種屬特異性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">同種蛋白不同物種，有著或大或小的差異。現在大部分商品化抗體都是以人源蛋白序列為模板重組蛋白或設計多肽抗原的，依據蛋白的同源狀況與其他種屬發作穿插</font> <font face="宋體">反響。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">需求參照闡明書注明的可反響種屬信息。一些稀有種屬很難找到抗體，能夠經過蛋白的序列比對，挑選同源序列免疫的抗體，不過一般這種狀況生產商不會受</font> <font face="宋體">理其關于質量的申述，假如能夠申請到免費的抗體樣品，則利于抗體挑選。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">3<font face="宋體">、試驗辦法特異性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">現在運用抗體的試驗辦法有許多，不同的運用辦法過程中，由于對蛋白樣品的處理方式不一樣，蛋白的含量有差異，對抗體的辨認表位和效價要求都是不一樣的。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">詳細的依據闡明書注明的產品應用辦法進行挑選。可是生產商一般不會將每個抗體都進行各種試驗的檢測，現在檢測比較多的僅僅</font>WB<font face="宋體">、</font><font face="Calibri">IHC</font><font face="宋體">、</font><font face="Calibri">IF</font><font face="宋體">等，假如針對詳細 的試驗辦法沒有找到適宜的抗體的，能夠結合蛋白序列剖析和抗體的抗原信息，挑選盡可能有作用的抗體。相同，申請到免費的抗體樣品是個很好的途徑。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">4<font face="宋體">、符號物的特異性：</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">一般根據試驗操作的試驗辦法不會運用帶有符號的一抗，比方</font>WB<font face="宋體">、</font><font face="Calibri">IHC</font><font face="宋體">等，都是經過二抗類的試劑符號到達成果出現的目的。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">以上的內容您把握的怎么樣了呢？假如您還有什么不明白的，不妨經過本網站</font>&ldquo;<font face="Calibri">QQ</font><font face="宋體">攀談&rdquo;聯絡本公司業務員，咱們將極力為您服務！上海酶聯生物一向致力于為廣闊高校、科研院所和企事業單位提供一流的科研試劑和完善的技術服務，滿意生物化學、分子生物學 、細胞生物學、免疫學等生物科技試驗需求。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">&nbsp;</span></p>